Genetic relationships of the Yucatan black hairless pig with Iberian breeds using single nucleotide polymorfisms / Relações genéticas de porcos pretos calvos de Yucatán com raças ibéricas usando Polimorfismo de nucleotídeos isolados

To conduct ex-situ creole pig conservation programs, it is essential to determine which breeding animals will be used, preferentially those with a more significant Iberian genetic component to preserve their origin. This study used a Yucatan black hairless pigs (YBHP) subpopulation to estimate its genetic diversity and population structure. One hundred four adult pigs were selected for the absence of hair, black skin (without spots), black hoof, and straight snout. The porcine-GGP-50K chip was used for SNP genotyping in YBHP, and information on Iberian and Yucatán hairless pigs from the United States (USYU) was taken from databases. All analysis was performed using PLINK v1.9 and v2.1 software. Inbreeding and fixation index values were lower in YBHP, with high observed heterozygosity and allogamy index values, which agree with those obtained in the populations of Canarias and Chato Murciano. According to the clusters generated by the "Genome-Wide Identity by State" analysis, four groups were identified, one of which included pigs from Guadyerbas, USYU, and YBHP. Between populations, YBHP was closely related to the hairless pigs from Guadyerbas, USYU, and Canarias. Principal component analysis showed the same result. According to the results obtained from the runs of homozygosity investigation, aimed to get pools consensus of regions of overlapping, 119 SNPs associated with genes and biological processes were identified. The BMP7 and NSUN2 genes were associated with epithelial cell differentiation, morphogenesis, and epithelial development. For nutrient metabolism: energy, the HADHA, PPARA, ADD1/SREBF1, and FAT 1genes were identified.(AU)

Para realizar programas de conservação ex-situ de suínos crioulos, é importante determinar quais animais serão criados, preferencialmente aqueles com maior componente de genética ibérica, para preservar sua origem. Uma subpopulação de porco preto calvo de Yucatán (YBHP) foi usada para estimar sua diversidade genética e estrutura populacional. Um total de 104 suínos adultos foram selecionados levando-se em consideração características como ausência de pelos, pele preta (sem manchas), casco preto e focinho reto. O painel GGP-50K foi utilizado para a genotipagem dos SNPs em animais YBHP, e informações de porcos sem pelos ibéricos e de Yucatán dos Estados Unidos (USYU) foram retiradas de bancos de dados. Todas as análises foram realizadas com o software PLINK v1.9 e v2.1. Os valores dos índices de endogamia e fixação foram menores em YBHP, com altos valores de índice de heterozigosidade e alogamia observados, que concordam com os obtidos nas populações de Canárias e Chato Murciano. De acordo com os clusters gerados pela análise "Genoma-Wide Identity By State", quatro grupos foram identificados, um dos quais incluiu porcos de Guadyerbas, USYU e YBHP. Entre as populações, YBHP estava intimamente relacionado com os porcos sem pelo de Guadyerbas, USYU e Canárias. A análise de componentes principais mostrou o mesmo resultado. De acordo com os resultados obtidos nas corridas de investigação de homozigose, visando obter consenso de pools de regiões de sobreposição, foram identificados 119 SNPs associados a genes e processos biológicos. Os genes BMP7 e NSUN2 foram associados à diferenciação de células epiteliais, morfogênese e desenvolvimento epitelial. Para metabolismo de nutrientes: energia, os genes HADHA, PPARA, ADD1/SREBF1 e FAT1 foram identificados.(AU)

Carrier frequency of autosomal recessive disorders (BC, BLAD, FXID and CVM) in Holstein cows in Jalisco, Mexico

The hereditary autosomal recessive disorders bovine citrullinemia (BC), bovine leukocyte adhesion deficiency (BLAD), factor XI deficiency (FXID), and complex vertebral malformation (CVM) have affected dairy cattle breeding significantly around the world. This study examined the carrier frequency of BC, BLAD, FXID, and CVM autosomal recessive disorders in Bos taurus Holstein cows bred in the Altos Norte region of the state of Jalisco, Mexico. We extracted DNA from 408 random samples of peripheral blood, and then used polymerase chain reaction (PCR) to identify insertion mutations for FXID, and PCR with restriction fragment length polymorphism (PCR-RFLP) for CVM, BC and BLAD. We visualized the PCR products using agarose gel electrophoresis stained with GelRed®. We found that 100% of wild-type (N/N) allele homozygous animals for genes CD18, ASS, and FXI were free of the mutations for BLAD, BC and FXID respectively. For gene SLC35A3 we estimated total carrier frequency of 10.3% and allele frequency of 5%.(AU)

Aspectos relevantes del bienestar del cerdo en tránsito / Relevant aspects of swine welfare in transit

Current globalization policies demand animal welfare standards on animal transportation. In spite of international tendencies to commercialize meat cuts while decreasing live animal transit, transport is still one of the major problems in terms of animal welfare, besides carcass and meat by-products' quality. The present review analyzes, in general terms, the different definitions on animal welfare and factors affecting pig welfare in transit. Several case studies are referred to as examples, showing the animal response to stress during transport and its effects on both meat quality and the economic impact. In addition, legal requirements and Mexican regulations for pig transportation are also described. Information in regard to swine stress and welfare in transit, considers a number of factors that alter the animal metabolic homeostasis with subsequent negative effects on pork quality. It is conclude that knowledge on basic animal behavioral and physiological needs during transport, as well as a suitable training of personnel, are necessary for reducing animal welfare problems. Last but not least, some recommendations on handling practices are given in order to improve swine welfare during transit.

En el marco actual de la globalización, hablar de transporte animal conlleva realizar prácticas que mejoren el bienestar animal, aunque existe la tendencia mundial de incrementar el mercado de la carne en cortes, así como disminuir el comercio de animales vivos, el transporte constituye uno de los factores más preocupantes en términos del bienestar animal, además del impacto en la calidad de la canal y los subproductos respectivos. En la presente revisión se analizan, en términos generales, las distintas definiciones de bienestar animal, así como los factores más importantes que alteran el bienestar de los cerdos durante el transporte; se describen los requisitos legales y la normatividad mexicana para el transporte de animales, así como varios estudios de la respuesta fisiológica del animal durante condiciones adversas del transporte, se señalan, además, las repercusiones sobre la calidad de la carne y su impacto económico. La información con respecto al estrés de los cerdos y su bienestar durante el transporte, considera numerosos factores que alteran el equilibrio homeostático animal y que propician efectos negativos sobre calidad de la carne. Se concluye que tanto el conocimiento de la biología de la especie, como un entrenamiento del personal, son necesarios para disminuir los problemas de bienestar. Finalmente, se recomiendan algunas prácticas derivadas de los hallazgos presentados, con el fin de que se mejore el bienestar de los cerdos en tránsito.

Efecto de genes candidatos sobre características reproductivas de hembras porcinas / Effect of Candidate Genes on Reproductive Traits of Sows

Se estudiaron genes candidatos para tamaño de la camada en 300 hembras porcinas Yorkshire-Landrace; ESR, PRLR, RBP4 y FUT1. Las hembras fueron agrupadas en dos niveles de producción (NP): nivel alto (NA) y nivel bajo (NB). Utilizando Ji cuadrado se analizaron las frecuencias génicas y genotípicas. Empleando análisis de varianza con un modelo de efectos mixtos, para lechones nacidos totales (LNT), nacidos vivos (LNV), peso de la camada al nacimiento (PNAC) y destete (PAJ21), lechones destetados (LD) y valor de cría de la progenie de la cerda (BVSP), se compararon las medias con contrastes ortogonales. Las hembras con alta productividad se asociaron con una mayor frecuencia del alelo B del gen ESR (P < 0,05). Las diferencias fueron de 0,4 LNV, 0,3 LD, 2,9 Kg de PAJ21 y 8,6 puntos de BVSP a favor del genotipo AB del gen ESR (P < 0,05) sin considerar el NP, no se detectaron animales homocigotos BB. Las frecuencias génicas y genotípicas del gen PRLR no se relacionaron con el NP (P > 0,05), no hubo diferencias (P > 0,05) entre los genotipos AA, AB y BB sin considerar el NP ni dentro del mismo NP. En el gen RBP4 la frecuencia del alelo A y del genotipo AA fue más alta en hembras con NA (P < 0,05), no se detectaron animales con genotipo BB. Las hembras con genotipos AA tuvieron más 0,5 LNT; 0,5 LNV; 0,6 Kg de PNAC; 2,6 Kg de PAJ21 y 3,2 puntos de BVSP que el genotipo AB (P < 0,05), sin considerar el NP. La frecuencia del alelo G y del genotipo GG del gen FUT1 fue mayor en el nivel de productividad alto (P < 0,05). El genotipo GG fue superior al genotipo AG con más 0,6 LNV; 0,8 Kg de PNAC; 3 Kg de PAJ21 y 3,9 puntos de BVSP (P < 0,05) sin considerar el NP.

Candidate genes were studied for litter size in 300 sows Yorkshire-Landrace; ESR, PRLR, RBP4 y FUT1. The sows were grouped in two levels of production (LP): high level (HL) and low level (LL). Using Chi-Square test the alleles and genotypic frequencies were analyzed. Employing analysis of variance with an mixed model effects for the total number born (TNB), number of piglets born alive (NBA), number of piglets alive at weaning (NW), total weight of piglets born (WTNB), total weight of piglets alive at weaning (WNW) and breeding value sow productivity (BVSP). The means were compared by orthogonal contrasts. The sows with high production were associated with a higher frequency of B allele of ESR gene (P < 0.05). The differences were of 0.04 NBA, 0.3 NW, 2.9 WNW kg and 8.6 BVSP points to favor of AB genotype of ESR gene (P < 0.05) without considering the LP and no homozygous BB animal was detected. The alleles and genotypic frequencies of PRLR gene were not related with the LP (P > 0.05), did not have differences (P > 0.05) between the genotypes AA, AB and BB without considering the LP, neither within of same LP. In the RBP4 gene the frequency of A allele and the AA genotype was higher in sows with HL (P < 0.05), no homozygous BB animals were detected. The sows with AA genotype had 0.5 TNB, 0.5 NBA, 0.6 WTNB kg, 2.6 WNW kg and 3.2 BVSP points more than sows with AB genotype (P < 0.05), without considering the LP. The frequency of G allele and GG genotype of FUT1 gene was higher in the HL (P < 0.05). The GG genotype was higher than AG genotype with 0.6 TNB, 0.8 WTNB kg, 3.0 WNW kg and 3.9 BVSP points more (P < 0.05), without considering the LP.